Fig. 2: Coverage of pan-Neisserial TbpB diversity using gonococcal TbpBs as vaccine antigens. | npj Vaccines

Fig. 2: Coverage of pan-Neisserial TbpB diversity using gonococcal TbpBs as vaccine antigens.

From: Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae

Fig. 2

A Heat map depicting ELISA-based reactivity of rabbit antiserum against a diverse panel of Neisserial TbpBs. Each row includes a different TbpB protein as a capture antigen, with the phylogenetic cluster indicated to the left. Each column represents either pre-immune or post 1, 2, or 3 vaccine dose serum for a single rabbit immunized with the vaccine formulation indicated below the x-axis. Increasing intensity indicates increasing immunoglobulin G specific for that TbpB variant. Background noise from empty vector control (maltose binding protein, MBP) has been subtracted. B hTf-binding to each TbpB as a plate control to quantification of properly folded (functional) TbpB. C Heat map depicting the ability of post 3 dose immune serum to block hTf binding to the TbpB capture antigen. Values are normalized to the signal obtained from no serum control. Each column represents a different rabbit immunized with the vaccine indicated below, or pooled pre-immune serum as a baseline control. Black represents a lack of hTf binding for the MBP empty vector control. AlOH aluminum hydroxide. Heat maps were generated using GraphPad Prism 10.1.1.

Back to article page