Fig. 3: Coverage of TbpB diversity using antigens formulated with alum versus emulsion adjuvants. | npj Vaccines

Fig. 3: Coverage of TbpB diversity using antigens formulated with alum versus emulsion adjuvants.

From: Rational selection of TbpB variants yields a bivalent vaccine with broad coverage against Neisseria gonorrhoeae

Fig. 3

A Heat map depicting the reactivity of rabbit antiserum against a broad ELISA panel of Neisserial TbpBs. Each row includes a different TbpB capture antigen, with the phylogenetic cluster indicated to the left. Each column represents either pre-immune or post 1, 2, or 3 dose serum for a single rabbit immunized with the vaccine indicated below the x-axis. Increasing intensity indicates increasing immunoglobulin G specific for that TbpB variant. Background noise from the empty vector (MBP) has been subtracted. B Heat map depicting the ability of post 1, 2, and 3 dose immune serum to block hTf binding by the TbpB capture antigen. Values are normalized to the signal obtained from no serum control. Each column represents a different rabbit immunized with the vaccine formulation indicated below. Black represents a lack of hTf binding for the MBP empty vector control. AlOH aluminum hydroxide. Heat maps were generated using GraphPad Prism 10.1.1.

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