Fig. 4: Coverage of clinical gonococcal isolates using gonococcal TbpBs as vaccine antigens.

A Heat map depicting the reactivity of rabbit antiserum against a broad ELISA panel consisting of heat-inactivated whole bacteria. Each row is a different gonococcal isolate, with the originating collection indicated to the left. Each column represents either pre-immune, or post 1, 2, or 3 dose serum for a single rabbit immunized with the vaccine indicated below the x-axis. AlOH, aluminum hydroxide. B hTf-binding to each isolate is used as a readout for surface expression of the TbpAB receptor. Heat maps were generated using GraphPad Prism 10.1.1. Arrows indicate strains that were used for serum bactericidal assays shown in Fig. 5.