Fig. 1: Construction and expression of the GPCv1 and GPCv2.
From: Structure-guided design of a prefusion GPC trimer induces neutralizing responses against LASV
a Linear schematic of the GPCv1 and GPCv2 constructs. A G4S linker was inserted between GP1 and GP2; T4fib was fused to the C terminal. b A molecular representation of GPCv2. c A representative elution chromatograph of PBS (gray dotted line) and GPCv1 (blue line) as well as GPCv2 (red line). d SDS-PAGE analysis of GPC v1 and GPCv2 (left: −DTT; right: +DTT). e A comparison of the N-glycosylation profile of GPCv1 and GPCv2. The percentage of each group is shown for each glycosite in the pie chart, and the proportion of each glycoform is shown in the bar chart. f Characteristics of N-glycosylation sites. The diagram illustrates the structural schematic of the modified GPCv2. The orange parts represent the glycosylation sites on the 7UOT structure, the yellow ones show the sites simulated from the 5vk2 structure, and the green ones indicate the glycosylation sites on GPCv2.
