Fig. 2: mRNA gE constructs in vitro screening and candidate selection. | npj Vaccines

Fig. 2: mRNA gE constructs in vitro screening and candidate selection.

From: A highly stable lyophilized mRNA vaccine for Herpes Zoster provides potent cellular and humoral responses

Fig. 2

mRNA vaccine candidates generated were tested in HeLa cells. A total of 50 ng of RNA were transfected and analyzed 24 h later through indirect immunofluorescence assay (IFA). Cells were fixed, permeabilized and percentage positive cells and mean fluorescence intensity (MFI) were measured using a specific antibody against the VZV gE protein. a Percentage of gE positive cells and (b) mean fluorescence intensity (MFI) of membrane anchored mRNA VZV gE constructs were measured for two different codon optimized sequences based on different GC content (Co1 and Co2). c Percentage of gE positive cells and (d) MFI of soluble mRNA VZV gE constructs was measured for two different codon optimized sequences based on different GC content (Co1 and Co2).

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