Fig. 5: Total and cell surface in vitro expression assay (IVE) of selected mRNA VZV gE vaccine candidates in HEK 293T cells. | npj Vaccines

Fig. 5: Total and cell surface in vitro expression assay (IVE) of selected mRNA VZV gE vaccine candidates in HEK 293T cells.

From: A highly stable lyophilized mRNA vaccine for Herpes Zoster provides potent cellular and humoral responses

Fig. 5

In vitro expression of mRNA VZV LNP formulated material for the three proposed vaccines candidates gE WT_Co2, ms5_Co1 and ms6_Co2 was measured by flow cytometry in HEK 293T cells and whole gE expression levels (a) or gE levels in the cell surface (b) were detected using a specific antibody against VZV gE. EC50 values (ng/well) were calculated from each curve and are shown in a table together with the (%) positive cells at 31 ng dose for both conditions tested.

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