Fig. 7: Stability and immunogenic profile of lyophilized mRNA material over time. | npj Vaccines

Fig. 7: Stability and immunogenic profile of lyophilized mRNA material over time.

From: A highly stable lyophilized mRNA vaccine for Herpes Zoster provides potent cellular and humoral responses

Fig. 7

In vitro expression (IVE) of frozen and lyophilized material for gE WT Co1 candidate was measured by flow cytometry in HEK 293 T cells at initial timepoints (a) as well as after 12 months (b) and 24 months (c). Whole gE expression levels were detected using a specific antibody against VZV gE. EC50 values (ng/well) were calculated from each curve and are shown in a table together with the (%) positive cells at 31 ng dose for all three conditions tested. Blood from mice previously immunized with 1 µg dose of Shingrix® or VZV gE WT Co1 (frozen or lyophilized) mRNA material preserved for 0 months (d), 12 months (e) or 24 months (f) was collected via submandibular route and gE-specific IgG antibodies (µg/ml) were measured by Luminex on day 76.

Back to article page