Fig. 3: Multistep growth curve of recombinant viruses compared to Georgia 2007/1. | npj Vaccines

Fig. 3: Multistep growth curve of recombinant viruses compared to Georgia 2007/1.

From: Deletion of B125R increases protection induced by a genotype II African swine fever vaccine candidate

Fig. 3

Purified PBMs were infected with the following viruses: (i) Georgia 2007/1, (ii) GeorgiaΔDP148RΔK145RΔEP153R-CD2v_mutQ96R/K108D (GΔDKE-CmutQ96R/K108D), the previous vaccine virus, (iii) GeorgiaΔDP148RΔK145RΔEP153R-CD2v_mutQ96R/K108D- NoFP (GΔDKE-CmutQ96R/K108D-NoFP), the previous vaccine virus after removing the fluorescent reporter markers, TagRFP-T and mNeonGreen, and (iv) GeorgiaΔB125RΔDP148RΔK145RΔEP153R-CD2v_mutQ96R/K108D (GΔBDKE-CmutQ96R/K108D), the current vaccine candidate with B125R gene deleted, at MOI 0.01 in triplicates. Viruses were harvested from both cells and supernatants at different time points and titrated on PBMs in quadruplicates. Multistep growth curves of the gene-deleted viruses and wild-type Georgia 2007/1 are shown over the course of 5 days, where Day 0 represents the inoculum (x-axis). Virus titres are shown on a log 10 scale on the y-axis as TCID50/mL. Two-way ANOVA with Dunnett’s multiple comparisons test was performed to evaluate the differences between the recombinant viruses and the wildtype virus. Significant differences are represented by asterisk where * is p < 0.05; the colour of the asterisk represents the respective virus.

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