Fig. 4: Experimental timeline.

The timeline for the 2 pig immunisation experiments is shown. Groups X, Y and D were primed and boosted with GeorgiaΔBDKE-CmutQ96R/K108D at different doses, 103, 104, and 102 TCID50 in 1 mL PBS or L2-RPMI intramuscularly, respectively. Pigs in Group C were immunised with 104 TCID50 without a booster dose. Two groups of non-immune pigs (Groups AA and F) were challenged in parallel to the immunised pigs (Groups X, Y, D, and C). In experiment 2, pigs in Group E were immunised at 104.5 TCID50 and were culled at 23 dpi, where † denotes the culling of Group E.