Fig. 9: Levels of infectious ASFV in whole blood from pigs.

Levels of viremia were determined by titrating virus in PBMs from the whole blood of pigs immunised and boosted with GΔBDKE-CmutQ96R/K108D at different doses a 103 (Group X), b 104 (Group Y), and c 102 (Group D), d pigs immunised (104)without a booster dose (Group C) and g pigs immunised with 104.5 TCID50 dose (Group E). Viremia for the non-immune, challenge control groups was measured following challenge with Georgia 2007/1 isolate. Results for the control Group AA in experiment 1 are shown in (e) and Group F for experiment 2 in (f). The dashed line on the y-axis represents the cut-off below which accurate measurement of infectious virus per mL blood was not obtained. Any value below this indicates the isolation of infectious ASFV but the exact titres cannot be determined. Results for Group E were below this level (g).