Fig. 2: Results of relative accuracy, intermediate precision specificity, and linearity tests.

a Relative accuracy refers to the degree to which the measured relative potency is close to the true value within a specified range, generally expressed as relative bias. b Intermediate precision refers to the geometric coefficient of variation (GCV) of relative potency measurements at each potency level. c specificity refers to relative bias of measured values between individual antigens and four mixed antigens at concentrations of 100, 400, and 1000 ng/ml with a ratio of 1:1:1:1. d–g linearity. Tests were performed by four experimenters at two different times, using five concentrations in the range of 50–200% (The concentration range of HPV6, HPV16 and HPV18 is from 10 to 40 ug/ml, The concentration range of HPV11 is from 5 to 20 ug/ml). Each point indicates the mean of eight replicates. Tests were performed using three concentrations in the range of 100 ng/ml to 1000 ng/ml.