Fig. 1: VLP purification schematic. | npj Vaccines

Fig. 1: VLP purification schematic.

From: Production of an immunogenic trivalent poliovirus virus-like particle vaccine candidate in yeast using controlled fermentation

Fig. 1

To compare yields of yeast-derived PV VLPs produced by either flask or bioreactor mediated expression, yeast pellets are processed in the same way. Pellets derived from equivalent culture volumes were mechanically disrupted at 40 kpsi ( ~ 275 MPa) and the cell lysates collected for further processing. Following removal of insoluble material, samples were treated with nuclease prior to a chemical precipitation step using PEG. The pelleted material was then resuspended and pelleted through 30% sucrose. The resulting pellet was resuspended in a small volume prior to sucrose density gradient ultracentrifugation. The resultant fractions were analysed for the presence of PV VLPs by ELISA.

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