Fig. 3: Comparison of PV2-SC6b and PV2-SC5a VLPs.

A Antigenicity of thermally stabilised PV2 VLPs purified from flask-based expression. Reactivity of gradient fractions using a PV2 specific monoclonal antibody for D antigen (MAb 1050) in ELISA. The pink dashed line represents the positive control, BRP. OD at λ = 492 nm is represented in arbitrary units. The figure is a representative example of three separate experiments for each variant. B Representative micrographs of PV2 VLPs purified from 10 L bioreactor material (Scale bar shows 200 nm). C Comparative analysis of PV2 VLP production in flask and bioreactor. Amount of purified D Ag produced per 100 mL culture as determined by ELISA using MAb 1050. OD at λ = 492 nm is represented in arbitrary units (n = 3) Means +/− standard deviation. Statistical analysis determined by two-tailed t test (**, p value > 0.01, ***, p value > 0.001). D Reactivity of purified PV2-SC6b and PV2-SC5a VLPs and BRP aliquots to D-antigen specific MAb 1050 in ELISA after incubation at different temperatures for 10 min, normalised to corresponding aliquot incubated at 4 °C. This figure is a representative example of two separate experiments for each variant.