Fig. 3: Spike-specific B-cell responses after bivalent COVID-19 booster vaccination. | npj Vaccines

Fig. 3: Spike-specific B-cell responses after bivalent COVID-19 booster vaccination.

From: Bivalent mRNA booster vaccination recalls cellular and antibody immunity against antigenically divergent SARS-CoV-2 spike antigens

Fig. 3

A Representative staining of antigen-specific memory B cells (CD19+IgD−) at baseline and 28 days post-vaccination. Two fluorochrome-conjugated spike probes were used to differentiate B-cell populations that were either specific for Ancestral (AN only), Omicron BA.1 (BA.1 only) or both spike proteins (AN+BA.1+). B Frequencies of antigen (Ag)-specific IgD− CD19+ B cells at days 0 and 28. C Proportion of IgG+ (solid bars), IgA+ (open bars), and IgM+ (checked bars) cells in AN, BA.1, or AN+BA.1+ MBC populations. D Proportion of activated CD27+ CD21− cells among MBC populations. E Correlation between baseline frequencies of AN+BA.1+ B cells and neutralisation titres against ancestral and Omicron BA.1 viruses at D28. N = 31 for all groups. Bold lines join the medians for each group. B-cell frequencies were analysed by the Wilcoxon signed-rank test. Correlations were analysed by Spearman r test. ***p < 0.001, ****p < 0.0001.

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