Fig. 1: Differential propensities of VZV and HPV subunit vaccines to elicit CD4+ and CD8+ T cell responses in C57BL/6 mice. | npj Vaccines

Fig. 1: Differential propensities of VZV and HPV subunit vaccines to elicit CD4+ and CD8+ T cell responses in C57BL/6 mice.

From: Repurposing anti-viral subunit and mRNA vaccines T cell immunity for intratumoral immunotherapy against solid tumors

Fig. 1

Splenocytes from naïve, VZV-vax and HPV-vax immunized mice following an intramuscular prime-boost regimen were analyzed after in vitro stimulation with overlapping peptide libraries derived from VZV gE and HPV L1 antigens or medium only. A, B Production of IFN-γ and TNF-α by (A) CD4+ and (B) CD8+ T cells was analyzed by flow cytometry and intracellular cytokine staining. Data are shown as mean percentage of positive CD4+ or CD8+ T cells ± standard error of the mean (SEM). C IFN-γ, IL-10, CCL4, IFN-α, CXCL9, CXCL10, TNF-α, IL-6, VEGF, IL-4, CCL3, CCL2, and GM-CSF production was measured using a bead-based multiplex immunoassay in supernatants of splenocyte cultures stimulated for 48 h with overlapping peptide libraries derived from the VZV gE and HPV L1 antigens. Heatmap represents the mean Z-score of the amount of each analyte in pg/ml in supernatants (n = 3 per group).

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