Fig. 6: Blockade of B7-H3 enhanced systemic antitumor immunity to inhibit lung metastasis.
a The splenocytes were harvested for H2-Kd-restricted mE2f8 neopeptide stimulation. Representative images of IFNγ+ ELISPOT from splenocytes are shown. *p < 0.05 and ***p < 0.001. One-way ANOVA. b The splenocytes were harvested for H2-Kd-restricted mMtch1 neopeptide stimulation. Representative images of IFNγ+ ELISPOT from splenocytes are shown. *p < 0.05 and ***p < 0.001. One-way ANOVA. c The splenocytes were harvested for H2-Kd-restricted mGlud1 neopeptide stimulation. Representative images of IFNγ+ ELISPOT from splenocytes are shown. *p < 0.05 and ***p < 0.001. One-way ANOVA. d The splenetic T cells were harvested and incubated with CT26 cells for 9 h. The cytotoxicity ability was evaluated by a CCK8 assay. *p < 0.05. e Scheme and regimen of AAV vaccination, radiotherapy and anti-B7-H3 antibodies. A total of 3 × 105 CT26 cells and 1 × 105 CT26 were subcutaneously and intravenously inoculated into the right leg of BALB/c mice, and AAV (1 × 108 vg) particles were intramuscularly injected on days 5, 12, 19 and 24. Local radiotherapy (5 Gy) of the right leg was conducted on days 11, 14 and 17. Anti-B7-H3 antibodies (100 μg/mouse) were administered intraperitoneally on days7, 10, 13 and 16. f Representative images of lung metastatic tumors were evaluated. The quantification of the lung weight is shown (n = 3). *p < 0.05. One-way ANOVA. g The quantification of the lung metastasis index is shown (n = 3). *p < 0.05. One-way ANOVA.
