Fig. 1: Experimental design of T-cell immune responses. | npj Vaccines

Fig. 1: Experimental design of T-cell immune responses.

From: Cellular immune signatures and differences of four porcine circovirus type 2 vaccines to heterologous PCV2d infection

Fig. 1

Thiry SPF pigs were divided into six groups (5 pigs/group): Mock (tangerine), PCV2d (red), G1 (green), G2 (mustard), G3 (blue), and G4 (purple) group. The G1-G4 groups were vaccinated with different vaccines, while the PCV2d and Mock groups served as negative controls. At 28 dpv, the G1-G4 and PCV2d groups were challenged with PCV2d, while the Mock group remained unchallenged. Heparinized blood was collected weekly (0, 7, 14, 21, 28 dpv, and 7, 14 dpc) to prepare peripheral blood mononuclear cells (PBMCs) for different immunological assays at indicated time points. All pigs were euthanized at 42 dpv (14 dpc), and spleen and inguinal lymph node (ILN) were collected to isolate mononuclear cells (MNCs) for several immunological tests. This figure and all its elements were originally created by the authors using Adobe Illustrator and Microsoft PowerPoint without adaptation of copyrighted material.

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