Fig. 4: The multifunctional CD8 T-cell responses in PBMCs after vaccination. | npj Vaccines

Fig. 4: The multifunctional CD8 T-cell responses in PBMCs after vaccination.

From: Cellular immune signatures and differences of four porcine circovirus type 2 vaccines to heterologous PCV2d infection

Fig. 4

At 14 and 28 dpv, intracellular cytokine staining of PBMCs was conducted after in vitro restimulation with PCV2-ORF2 antigen, with cRPMI-maintained cultures as blank controls. a Representative graphs for IFNγ/TNFα double-positive CD8 T cells in PBMCs from different groups at 14 and 28 dpv. Comparison of single-, double-, or triple-positive cytokine responses in CD8 T cells at 14 dpv (b), and 28 dpv (c) across distinct groups. Background-subtracted data are shown as scatterplots with individual samples (dots); the horizontal line represents the mean; error bars show standard deviations (SD); dashed black line denotes the limit of detection (LOD); Responders (top row) depicts the number of positives in diverse groups. P values were determined from an unpaired two-tailed t-test with Welch’s correction as appropriate.

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