Fig. 5: The dynamic changes of different memory T cell subsets in PBMCs after vaccination.
Prepared PBMCs were utilized to analyze the phenotypic changes in CD4 and CD8 T cells at 0, 14, and 28 dpv. (a) Representative dot plots of CD27 and CD8α expression on CD4 T cells in PBMCs from different groups at 0, 14, and 28 dpv. The values in the quadrants indicate the percentages of each CD4 T cell subset, with red-shaded areas depicting the percentage of CD4 TEM cells. Dynamic changes of CD4 TEM cells (b), CD8 TEM cells (c), and CD8 TEMRA cells (d) in PBMCs from different groups. Groups are color-coded: Mock in tangerine, G1 in green, G2 in mustard, G3 in blue, and G4 in purple. Gray symbols indicate individual samples, connected by gray lines. Color-coded bold lines represent the mean at each time point. Statistical significance of different groups at each time point was determined using an unpaired two-tailed t-test with Welch’s correction as appropriate and is shown as “Intergroup Comparison” on the top panel, color-coded based on the groups compared. 0.05 ≤ P values < 0.1 are shown as bold; P values < 0.05 are indicated by bold underline; P values < 0.001 are denoted as 0.000. Asterisks indicate significant intragroup differences between points in time (*P < 0.05, **P < 0.01, ***P < 0.001) as detected by paired t-tests.
