Fig. 9: PCV2-ORF2-specific T-cell cytokine response in tissues at necropsy. | npj Vaccines

Fig. 9: PCV2-ORF2-specific T-cell cytokine response in tissues at necropsy.

From: Cellular immune signatures and differences of four porcine circovirus type 2 vaccines to heterologous PCV2d infection

Fig. 9

The IFNγ-ELISPOT and intracellular cytokine staining assays were performed to assess PCV2-ORF2-specific T-cell cytokine response in the spleen and inguinal lymph node (ILN). a Representative images of ELISPOT wells in the spleen and ILN from each group. b PCV2-ORF2-specific IFNγ-SC responses in the spleen and ILN across groups. c Representative dot plots for IFNγ/TNFα/IL-2 triple-positive CD4 and CD8 T cells in the spleen from distinct groups. Comparison of single-, double-, or triple-positive cytokine responses in splenic CD4 T cells (d) and CD8 T cells (e) across groups. Colored dots represent background-subtracted data from individuals; horizontal lines display means; error bars indicate standard deviations. The dotted black line denotes the limit of detection (LOD). Responders (top row) show the number of pigs with positive responses. Asterisks denote significant differences (*P < 0.05, **P < 0.01) between the indicated groups as determined by an unpaired two-tailed t-test with Welch’s correction as appropriate.

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