Fig. 3: Genetic requirements across distinct immunization conditions.

a Experimental strategy for the expanded TnSeq screen. n = 2–5 mice per group. b Heatmaps showing log₂ fold-change in transposon insertion count relative to naïve mice for the Esx-1 genes (left panels) and Mce1 genes (right panels) for each immunization condition. Asterisks indicate adj. p < 0.05 by the Transit resampling pipeline, as detailed in the “Methods.” c Box plots showing log₂ fold-change in transposon insertion count for Esx-1 and Mce1 genes, relative to naïve mice. Significance determined by the Friedman test with Dunn’s multiple comparison to the BCG-SQ condition.