Table 2 List of primer sequences, final primer concentrations and temperature conditions of the qPCRs
Gene | Primer concentration (µM) | Amplification temperature (°C) | Forward sequence | Reverse sequence | Sequence source | NCBI reference number of the transcript |
|---|---|---|---|---|---|---|
Rpl18 | 0.25 | 58 | GTTTATGAGTCGCACTAACCG | TGTTCTCTCGGCCAGGAA | Zivcec et al. Journal of Immunological methods (2011) | XM_005084699.3 |
Ywhaz | 0.125 | 60 | GAAGCGGAAGCAGGAGAAG | TATTTGTGGGACAGCATGGA | Self-developed, using Primer 3 software (on-line) | AY569127.1 |
Il6 | 0.5 | 62 | CCTGAAAGCACTTGAAGAATTCC | GGTATGCTAAGGCACAGCACACT | Self-developed, using Primer 3 software (on-line) | XM_005087110.2 |
Il13 | 0.075 | 60 | GCACTCTGGGTGACCGTAGT | GCCCTCTGGTCTTGTGTGAT | Self-developed, using Primer 3 software (on-line) | XM_005067910.3 |
Tnf | 0.25 | 60 | TGAGCCATCGTGCCAATG | AGCCCGTCTGCTGGTATCAC | Espitia et al. BMC Immunology 2010 | XM_005086799.3 |
Ifng | 0.125 | 63 | TGTTGCTCTGCCTCACTCAGG | AAGACGAGGTCCCCTCCATTC | Espitia et al. BMC Immunology 2010 | NM_001281631 |
Il4 | 0.075 | 60 | ACCGAGATGGTCGTACCAGA | CACAGGGTCACCTCATGTTG | Self-developed, using Primer 3 software (on-line) | XM_005067769.2 |
Il2 | 0.125 | 60 | AGTGCACCCACTTCAAGCTC | GCCTTCTTGGGCATGTAAAA | Self-developed, using Primer 3 software | NM_001281629.1 |
