Table 3 Cycling conditions for the qPCRs

From: Hamsters immunized with formalin-inactivated SARS-CoV-2 develop accelerated lung histopathological lesions and Th2-biased response following infection

Stage

Repetitions

Temperature

Time

1

1

95.0 °C

10:00

2

40

95.0 °C

0:15

XX.0 °Ca

1:00

3b (Dissociation)

1

95.0 °C

0:15

60.0 °C

1:00

95.0 °C

0:15

60.0 °C

0:15

  1. aThis temperature is primer-specific and was optimized for each primer set. The relevant temperatures are listed in Table 2 (Amplification temperature).
  2. bStep 3 was used to generate dissociation curves. Quality of the PCRs and the generated fragments were evaluated per PCR run based on the melting curves. Samples that had PCR products with melting temperatures different than the expected PCR product were excluded from the analysis.
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