Fig. 2: Analysis of the four sE2 glycoforms. | npj Vaccines

Fig. 2: Analysis of the four sE2 glycoforms.

From: Glycoengineering of the hepatitis C virus E2 glycoprotein improves biochemical properties and enhances immunogenicity

Fig. 2

A SDS-PAGE analysis of CHO sE2, geCHO.sE2.1, geCHO.sE.7, and HEK sE2 under reducing conditions. B Western blot detection of the purified proteins under reducing conditions. C Western blot detection of the purified proteins under non-reducing conditions. Under these conditions, each sample migrated as two distinct species. We have therefore inserted markers to the right of the blot indicating where each species migrates. The anti-E2 antibody HCV1 antibody was used at a concentration of 5 μg/mL for the Western blots. The SDS-PAGE and western blots shown are representative examples from 3 independent experiments.

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