Fig. 3: Treg depletion enables in vivo lytic CD8+ T cell responses to Qβ-A4Y vaccination.
From: Regulatory T cells define affinity thresholds for CD8+ T cell tumor infiltration
A An illustration depicting the vaccination regimen administered following transplantation of B16F10p33 tumors, combined with intravenous injection of anti-CD25 mAb for effective depletion of Tregs. Four groups were organized: Qβ, Qβ-A4Y, Qβ-A4Y/Treg depl, and Treg depl alone serving as an additional control. B Gating strategy of FOXP3+ T cells within CD4+ CD25+ T cells in the blood, measured 5 days post-vaccination and Treg depletion. C Percentage of FOXP3+ T cells within CD4+ CD25+ T cells in the blood in the designed groups. D Tumor volume mm3. E Tumor volume mm3 of individual mice. F Density of A4Y+ cells within CD8+ T cells in the tumors. G Density of IFN-γ producing A4Y+ cells within the CD8+ T cell population in the tumor. Stimulation was conducted using the corresponding peptide. H Representative flow cytometry plots illustrating the total number of A4Y+ cells within CD8+ T cells acquired from each tumor. Statistical analysis with one-way ANOVA. The sample size for each group was n = 5, 6 or 9.
