Fig. 4: Local immune response in BALFs and lungs in BALB/c mice immunized with rNA-N1-MPP vaccine adjuvanted with BDX100 or BDX301 adjuvants.

a Percentage of memory B-cells and b activated B-cells in the lungs of vaccinated mice 10 days after the booster immunization (n = 5). c Level of IgA and IgG in the BALFs obtained from the immunized animals. Dots represent individual AUC values (n = 5); bars represent average values. d Flow cytometry plots showing the percentage of different B-cell subpopulations in the lungs of immunized mice (gated on live CD3^‒CD19⁺CD38⁺GL7^‒ cells). e Representative flow cytometry plots showing the percentage of tissue-resident memory T-cell subpopulations in the lungs of immunized mice (gated on live CD3⁺CD19^‒CD4/CD8⁺ cells). Plots were produced by combining data from each separate sample into one file for each group (d, e). f–m CD4⁺ and CD8⁺ EM (CD44⁺CD62L^‒), T_RM (CD103⁺CD69⁺, CD103^‒CD69⁺), and CD103⁺CD69^‒ T-cell subpopulation percentage of the total number of CD4⁺ or CD8⁺ T-cells in the lungs of mice 10 days after the booster immunization (n = 5). Groups were compared using ANOVA followed by the Tukey posthoc test. P-values for the groups with statistically significant differences are shown on the plots.