Fig. 5: T-cellular immune response to the rNA-N1-MPP vaccine adjuvanted with BDX100 or BDX301 in C57BL/6 mice. | npj Vaccines

Fig. 5: T-cellular immune response to the rNA-N1-MPP vaccine adjuvanted with BDX100 or BDX301 in C57BL/6 mice.

From: Immunogenicity and protective efficacy of an intranasal neuraminidase-based influenza vaccine with bacterial cell membrane-derived adjuvants

Fig. 5

a Flow cytometry plots showing the percentage of IL-2- and TNFα-producing CD4+ EM T-lymphocytes in mouse lungs and spleens 10 days after the booster immunization (gated on live CD3+CD19‒CD4+CD62L‒CD44+ cells). Cells were incubated with overlapping peptides, covering the whole sequence of N1 NA-protein for 6 h in the presence of Brefeldin A and co-stimulatory anti-CD28 antibodies. Plots were produced by combining data from each separate sample into one file for each group. b, c Percentage of different cytokine-producing cell populations within the total CD4+ EM T-cell subset after background subtraction in the lungs (b) and spleen (c). Average and standard error (SE) values are shown. Statistical analyses were performed using one-way ANOVA with Tukey posthoc test. P-values for the groups with statistically significant differences are shown on the plots.

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