Fig. 1: Expression levels of p53 and mature DC markers in Ad-p53 DCs.

A Schematic illustration of the experimental protocol of preparation of dendritic cells (DCs) transduced with the replication-deficient, wild-type p53-expressing adenovirus Ad-p53 (Ad-p53 DCs). Bone marrow-derived cells isolated from the femur of BALB/c mice were incubated with GM-CSF (40 ng/mL) and IL-4 (10 ng/mL) for 5 days and then infected with Ad-p53 (100 MOI) for 2 days to obtain Ad-p53 DCs. Figures were generated using BioRender. B Expression of human p53 mRNA in control DCs and DCs infected with DL312 (100 MOI) or Ad-p53 (100 MOI) for 48 h. GAPDH was used as a control gene. C Representative photographs of expression of human p53 protein in DCs infected with Ad-p53 (100 MOI) for 72 h. Scale bars, 100 μm. D Expression of mature DC markers in Ad-p53 DCs. Control DCs and Ad-p53 DCs were subjected to flow cytometry for analysis of CD11c, CD86, MHC class-II (MHC-II), CD103, and CCR7 expression. The mean fluorescence intensity (MFI) for each protein was determined by calculating the difference between the MFI of the antibody- and control IgG-treated cells. Data are expressed as mean ± SD (n = 3). Statistical significance was determined using one-way ANOVA followed by Tukey’s comparison test. ***P < 0.001; ****P < 0.0001.