Fig. 2: Early-life HB-alum immunization results in the development of Th2-polarized immune responses to bystander OVA exposure. | npj Vaccines

Fig. 2: Early-life HB-alum immunization results in the development of Th2-polarized immune responses to bystander OVA exposure.

From: Rational adjuvant selection for the neonatal period shapes unique and lasting immune polarization in mice

Fig. 2

A Mice (n = 8–10) were immunized at DOL7 or 56 with HB-alum and received 4 weekly i.n. exposures to OVA from weeks 4–7. Mice were sacrificed 2 weeks after the final OVA exposure, and splenocytes were harvested and stimulated with OVA for 72 h. B, C Cytokine secretion was measured in cell culture supernatants. D, E Mice were immunized at DOL7 with HB-alum and received 4 weekly i.n. exposures to OVA during weeks 11–14. Mice were sacrificed 2 weeks after the final OVA exposure, and splenocytes were harvested and stimulated with OVA for 72 h. E OVA-specific cytokine secretion was measured in cell culture supernatants. Data are displayed as mean ± SEM. Statistical differences were determined using the Mann–Whitney test; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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