Fig. 1: Matrix-M accumulates inside lysosomes. | npj Vaccines

Fig. 1: Matrix-M accumulates inside lysosomes.

From: Matrix-M adjuvant triggers inflammasome activation and enables antigen cross-presentation through induction of lysosomal membrane permeabilization

Fig. 1

BMDCs were loaded with LysoTracker Red DND-99, washed and imaged for 30 minutes. Following the initial pre-bleaching image acquisition, cells were treated with BODIPY-labeled Matrix-M, Matrix-A, or Matrix-C at concentrations of 1, 2.5, or 5 μg/mL, and live-cell spinning-disk confocal imaging continued for an additional 120 minutes. A Representative images showing individual channels (LysoTracker, BODIPY), overlays, and the DIC (differential interference contrast) channel captured after each treatment (scalebar = 10 μm). B The fluorescence intensity kinetics of BODIPY fluorescence for the indicated Matrix concentrations. C Pearson’s correlation coefficients (PCC) for BODIPY and LysoTracker fluorescence over time. Data shown in B and C represent smoothed averages from at least three biological replicates ±SEM.

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