Fig. 4: Serum IgG response to vaccination. | npj Vaccines

Fig. 4: Serum IgG response to vaccination.

From: Evaluation of highly conserved Burkholderia pseudomallei outer membrane proteins as protective antigens against respiratory melioidosis

Fig. 4

a Timeline of mouse immunizations and tissue collection. Made with BioRender (https://BioRender.com/uxd6kaz). Serial dilutions of serum were probed for total IgG b or IgG1/IgG2c c via ELISAs coated with the indicated recombinant proteins. d The ability of serially diluted serum IgG to react to intact bacteria was measured with ELISA plates coated with Bpm strain Bp82. Endpoint titers were calculated as the highest dilution at which measured absorbance was higher than the average absorbance of the equivalently diluted naïve animal serum ± 3 standard deviations. Bacteria-specific IgG endpoint titers were compared via Kruskal–Wallis with Dunn’s multiple comparison test. (**) p < 0.01. Graphs made in GraphPad Prism.

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