Fig. 5: Mucosal IgA response to vaccination.

Serial diluted serum (a), 1:5 diluted BAL fluid (b), and 1:15 diluted supernatants from cultured NALT tissue c were probed for antigen-specific IgA. Endpoint titers were calculated as the highest dilution at which measured absorbance was higher than the average absorbance of the equivalently diluted naïve animal serum ± 3 standard deviations. BAL and NALT absorbance values were compared via two-tailed, unpaired Student’s t-tests. (**) p < 0.01, (****) p < 0.0001. Made with GraphPad Prism.