Fig. 6: Splenic lymphocyte response post vaccination. | npj Vaccines

Fig. 6: Splenic lymphocyte response post vaccination.

From: Evaluation of highly conserved Burkholderia pseudomallei outer membrane proteins as protective antigens against respiratory melioidosis

Fig. 6

a Flow cytometry gating strategy. b Total lymphocyte counts recovered from vaccinated animals. B cells = B220 + CD3-, CD4 T cells = CD3 + CD4 + CD8a-, and CD8 T cells = CD3 + CD4-CD8a + . CD44 and CD62L expression patterns of CD4 T cells c, d and CD8 T cells (e, f), measured via flow cytometry. Naïve cells = CD44-CD62L + , effector cells = CD44 − CD62L−, effector memory cells (TEM) = CD44 + CD62L−, and central memory cells (TCM) = CD44 + CD62L+. Total lymphocyte counts and proportions of CD44/CD62L-expressing T cells were compared via one-way ANOVAs with Dunnett’s multiple comparison correction. (*) p < 0.05, (**) p < 0.01. Made with GraphPad Prism.

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