Fig. 9: Measuring IgG cross-reactivity to heterologous Burkholderia species. | npj Vaccines

Fig. 9: Measuring IgG cross-reactivity to heterologous Burkholderia species.

From: Evaluation of highly conserved Burkholderia pseudomallei outer membrane proteins as protective antigens against respiratory melioidosis

Fig. 9

Serial dilutions of immunization serum were probed for IgG capable of binding to Bm strain CLH001, B. multivorans strain ATCC 17616, or B. cenocepacia strain K56-2 via ELISAs coated in the indicated bacteria. The Bpm strain Bp82 ELISA results from Fig. 4d are included for side-by-side comparison. Endpoint titers were calculated as the highest dilution at which measured absorbance was higher than the average absorbance of the equivalently diluted naïve animal serum ± 3 standard deviations. Endpoint titers were compared via Kruskal–Wallis with Dunn’s multiple comparison test. (*) p < 0.05, (**) p < 0.01, (***) p < 0.001. Made in GraphPad Prism.

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