Fig. 6: STS solves mAbs that bind to the FP region with varying FP interactions.
From: Immunofocusing on the conserved fusion peptide of HIV envelope glycoprotein in rhesus macaques
a FP-ELISA showcasing degree of FP interaction of the 8 different FP mAbs expressed from STS. b BLI curves of mAbs 05 (left) and 08 (right) interacting with trimers AMC016 (purple), BG505-CH505 + N241 (blue) and negative control BG505 MD39 link 14 (orange). c Colored EM maps of AMC016 (gp120 – dark gray, gp41 – light gray, glycans – green) in complex with RQk-FP-mAb 05 (heavy chain – orange, light chain – pale yellow), and PGT122 (pink). Bottom views showcase FP in purple. d RQk-FPA pAb model, when docked into the mAb 05 EM map, shows high degree of overlap of FP antibody and FP density, shows same angle of approach and N88 glycan interactions. e RQk-FPA pAb modelled FP regions (blue) shows poor overlap with mAb 05 EM map density for FP region (purple), showing slightly different modes of FP engagement between the two antibodies. f Antibody neutralization panel of RQk-FP mAbs 01, 03, 05, and 07 show >50.00 μg/mL concentration for PV neutralization, which is interpreted as non-neutralizing. PV panels include BG505 and related mutants panel and AMC016 (right), a CH505 PV panel (middle) and a FP-sensitive PV panel (right).
