Fig. 1: Designed antigens can be expressed and purified as monomers and displayed on ferritin nanoparticles.
From: A combined designed CSP and Pfs48/45 infection and transmission blocking vaccine for malaria
a Design of chimeric antigen as monomer and ferritin particle. CSPj5c and 17-4 are designed immunogens based on CSP and Pfs48/45 D3 proteins respectively. b Size-exclusion chromatography profile of CSPj5c-17-4 and 17-4-CSPj5c on Superose 6 Increase 10/300 GL column after nickel resin purification. c Size-exclusion chromatography profile of CSPj5c-17-4-ferritin and 17-4-CSPj5c-ferritin on Sepax SRT SEC-1000 column from Expi293F supernatant. d SDS-PAGE gel electrophoresis of purified monomeric protein and ferritin particle (reduced). e Negative-stain EM micrographs of purified nanoparticles displaying chimeric antigens with 2D classification averages (bottom) scale bar = 50 nm.
