Table 1 Molecular weight of monomeric fusion antigen and ferritin nanoparticle

From: A combined designed CSP and Pfs48/45 infection and transmission blocking vaccine for malaria

Antigen

Theoretical molecular weight (kDa), Monomer

Theoretical molecular weight (kDa), Nanoparticle (24 mer)

Molecular weight (kDa) determined by SEC-MALS

Molecular weight (kDa) determined by SEC-SAXS

Radius of Gyration, Rg (Ã…) determined by SEC-SAXS

CSPj5c-17-4

28.8

-

27.9 ± 0.26

40.2

31.86 ± 0.49

17-4-CSPj5c

28.8

-

26.9 ± 0.17

36.9

30.23 ± 0.53

CSPj5c-17-4-ferritin

48.4

1162

911 ± 3.78

1200

85.03 ± 0.50

17-4-CSPj5c-ferritin

48.4

1162

954 ± 4.59

1200

88.71 ± 1.36

  1. The theoretical molecular weights were calculated using Expasy ProtParam tool. The experimental molecular weight from SEC-MALS were determined using Wyatt ASTRA 8 software. Molecular weight from SEC-SAXS and the radius of gyration (Rg) with standard deviation were determined using SIBYLS SEC-SAXS Process GUI and ATSAS software suite.
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