Table 2 Binding kinetics and affinities to CIS43 antibody as determined by BLI
From: A combined designed CSP and Pfs48/45 infection and transmission blocking vaccine for malaria
Antigen | KD(M) x 10-9 | ka (1/Ms) x 105 | kd (1/s) x 10-3 |
|---|---|---|---|
CSPj5c | 0.84 ± 0.36 | 22.4 ± 0.24 | 1.89 ± 0.27 |
CSPj5c-17-4 | 1.08 ± 0.08 | 14.6 ± 0.05 | 1.59 ± 0.16 |
17-4-CSPj5c | 1.21 ± 0.09 | 13.1 ± 0.02 | 1.59 ± 0.13 |
Antigen-ferritin | KD(M) x 10-12 | ka (1/Ms) x 105 | kd (1/s) x 10-7 |
CSPj5c-ferritin | 1.80 ± 0.28 | 2.25 ± 0.03 | 4.08 ± 0.63 |
CSPj5c-17-4-ferritin | 1.84 ± 0.27 | 2.32 ± 0.09 | 4.24 ± 0.46 |
17-4-CSPj5c-ferritin | 1.88 ± 0.29 | 2.10 ± 0.03 | 3.94 ± 0.54 |
