Table 3 Binding kinetics and affinities to TB31F antibody as determined by BLI
From: A combined designed CSP and Pfs48/45 infection and transmission blocking vaccine for malaria
Antigen | KD(M) x 10−10 | ka (1/Ms) x 105 | kd (1/s) x 10−4 |
|---|---|---|---|
17-4 | 7.70 ± 0.81 | 9.76 ± 0.60 | 7.66 ± 1.33 |
CSPj5c-17-4 | 6.26 ± 0.70 | 11.8 ± 0.01 | 7.44 ± 0.92 |
17-4-CSPj5c | 6.21 ± 0.24 | 12.4 ± 0.01 | 7.74 ± 0.37 |
Antigen-ferritin | KD(M) x 10−12 | ka (1/Ms) x 105 | kd (1/s) x 10−7 |
17-4-ferritin | 2.95 ± 0.32 | 1.35 ± 0.05 | 3.96 ± 0.32 |
CSPj5c-17-4-ferritin | 2.10 ± 0.26 | 1.98 ± 0.04 | 4.18 ± 0.49 |
17-4-CSPj5c-ferritin | 1.55 ± 0.16 | 2.73 ± 0.06 | 4.23 ± 0.42 |
