Fig. 5: Migration of ARGs and ARB from wastewater to M. elatinoides. | npj Clean Water

Fig. 5: Migration of ARGs and ARB from wastewater to M. elatinoides.

From: Integrating livestock and aquatic plant towards mitigating antibiotic resistance transmission from swine wastewater

Fig. 5

a The absolute abundance of 16S rRNA gene and typical ARGs in the root of M. elatinoides at 0 d and 12 d. b The absolute abundance of 16S rRNA gene and typical ARGs in the shoot of M. elatinoides at 0 d and 12 d. c The growth of ARB in the LB agar plate containing specific antibiotics in the root of M. elatinoides at 0 d and 12 d. d The growth of ARB in the LB agar plate containing specific antibiotics in the shoot of M. elatinoides at 0 d and 12 d. e The amount of cultivatable ARB in the root of M. elatinoides at 0 d and 12 d. f The amount of cultivatable ARB in the shoot of M. elatinoides at 0 d and 12 d. g Experiment design for the migration of ARB based on an EGFP-labeling indigenous bacterium M. schleiferi S07::EGFP. h Representative inverted fluorescent microscopy images of green fluorescent M. schleiferi S07::EGFP in the hydroponic solution and different plant compartments of M. elatinoides at 0 d, 4 d and 12 d, respectively. SMX: Sulfamethoxazole; TET: Tetracycline; CHL: Chloramphenicol; CIP: Ciprofloxacin; KAN: Kanamycin; AMP: Ampicillin. “*”, “**”, and “***” denote significant differences between different treatments at different threshold of p < 0.05, p < 0.01, and p < 0.001, respectively.

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