Fig. 5: SHERLOCK lateral-flow detection of SAR-CoV-2 RNA with an internal RNase-contamination control.
From: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
a, Sequence of RNase reporter. Red lines indicate RNase A and RNase T1 cleavage sites. b, Predicted colorimetric outcomes for negative, positive and RNase-contaminated samples. c, Comparison of a conventional lateral-flow readout of SHERLOCK detection of SARS-CoV-2 RNA (left) and a lateral-flow strip with internal RNase-contamination detection (right), in the presence and absence of RNase A. d, Similar LoD obtained from conventional lateral-flow strips (left) and strips containing internal RNase detection (right), with SHERLOCK S gene detection of serially diluted SARS-CoV-2 RNA.
