Table 2 Sensitivity, specificity, predictive agreement and DOR characterizations of SHERLOCK detection of SARS-CoV-2 RNA compared with RT–qPCR
From: Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA
All samples | Samples within SHERLOCK LoD | ||
|---|---|---|---|
SHERLOCK lateral-flow readout | Sensitivity | 87.65% | 97.14% |
(95% CI) | (78.47–93.92) | (90.06–99.65) | |
Specificity | 100.00% | 100.00% | |
(95% CI) | (95.07–100.00) | (95.07–100.00) | |
PPA | 100.00% | 100.00% | |
(95% CI) | (94.94–100.00) | (94.72–100.00) | |
NPA | 87.95% | 97.33% | |
(95% CI) | (78.96–94.04) | (90.70–99.68) | |
ln(DOR) | 6.91 | 8.30 | |
(95% CI) | (4.05–9.76) | (5.25–11.40) | |
SHERLOCK fluorescence readout | Sensitivity | 96.30% | 100.00% |
(95% CI) | (89.56–99.23) | (94.87–100.00) | |
Specificity | 100.00% | 100.00% | |
(95% CI) | (95.07–100.00) | (95.07–100.00) | |
PPA | 100.00% | 100.00% | |
(95% CI) | (95.38–100.00) | (94.87–100.00) | |
NPA | 96.05% | 100.00% | |
(95% CI) | (88.89–99.18) | (95.07–100.00) | |
ln(DOR) | 8.10 | 9.94 | |
(95% CI) | (5.12–11.08) | (6.01–13.87) |
