Table 3 Attributes of the HC-FIA assay and a typical commercially available RT–qPCR kit for the detection of SARS-CoV-2 RNA
From: Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA
Principle | HC-FIA | RT–qPCR31 |
|---|---|---|
Pretreatment | Amplification free | Nucleic acid extraction and purification |
Assay time | Less than 1 h | 2–3 h |
Operating environment | On-site and on-demand detection | Requires specialized laboratories |
Requirements for operator | No need for professional technicians Operators can be easily trained | Requires trained technicians |
Equipment | Portable suitcase | Precision instruments, non-portable |
Throughput | 60 tests per houra | 96 tests per 4 h |
Application scenarios | Outpatient departments, emergency departments, customs, disease-control sites | Central laboratories |
Potential risk of pollution | No laboratory-contaminating product | Risk of contamination from amplicons |
