Fig. 1: CRISPR-powered gFET for amplification-free detection of single-nucleotide mutations. | Nature Biomedical Engineering

Fig. 1: CRISPR-powered gFET for amplification-free detection of single-nucleotide mutations.

From: Discrimination of single-point mutations in unamplified genomic DNA via Cas9 immobilized on a graphene field-effect transistor

Fig. 1

SNP-Chip, the next generation of CRISPR-powered gFET, with expanded monitoring of multiple electronic parameters, can detect single-nucleotide differences within unamplified DNA samples. Through the CRISPR–Cas9 ribonucleic protein complex on its surface, this technology can digitally detect SNPs without labels or amplification. The target-specific gRNA is designed to target single-nucleotide mutations relevant to two human disease models: SCD and ALS. Through real-time, multi-parameter and digital data acquisition, SNP-Chip can discriminate between unamplified genomic DNA samples in 40 min.

Back to article page