Extended Data Fig. 3: Analysis of lymphocytes in primary tumours.
From: Solid stress impairs lymphocyte infiltration into lymph-node metastases
a, Representative immunofluorescent staining of naïve lymph node (positive control, n = 9) and 4T1 primary tumour (14 days post-implantation, n = 4) stained concomitantly with anti-cytokeratin (green), anti-B220 (red), and anti-CD3 (white). b, Quantification of B220 + B cells and CD3 + T cells in 4T1 primary tumours (n = 4). c, Quantification of proliferating T cells based on CD3/Ki67 double-positive cells per 20x field of view in FTY720 or control treated mice: TDLN = non-metastatic tumour draining lymph node; MET = LN tissue remaining of a metastatic tumour draining lymph node (outside lesion) and metastatic tissue (inside lesion); L = metastatic lesion only in a metastatic tumour draining lymph node (inside lesion) Statistical analysis: Data plotted as the mean±s.e.m. Statistical significance was tested by 2-tailed unpaired Students’ t test (b) and by one-way ANOVA with Tukey’s Honestly Significant Difference post-hoc test (c).
