Extended Data Fig. 6: High-endothelial-venule wall-thickness in non-metastatic and metastatic lymph nodes.
From: Solid stress impairs lymphocyte infiltration into lymph-node metastases
a, Representative immunofluorescent staining of naïve lymph node, non-metastatic tumour draining lymph node and metastatic tumour draining lymph node with anti-cytokeratin (green) and anti-PNAd (red). DAPI (blue). Scale bars = 1272 µm. Inset below shows magnified region of interest from respective tiled lymph nodes. Scale bar = 50 µm. Four images were taken per group. b, Quantification of HEV wall thickness. Each point represents the average of 8 measurements per vessel. n = 4 animals for naïve and non-metastatic; n = 3 animals for metastatic. Data plotted as mean with 95% CI. Statistical significance was tested by one-way ANOVA with Tukey’s Honestly Significant Difference post-hoc test.
