Extended Data Fig. 10: ICAM-1 in lymph nodes experiencing solid stress.
From: Solid stress impairs lymphocyte infiltration into lymph-node metastases
a, Representative immunofluorescence staining of ICAM-1(red) and cytokeratin (green) in non-metastatic and metastatic LNs from mouse 4T1 model. The number of images taken is reflected in the n values for b. b, Quantification of ICAM-1 in non-metastatic (n = 3) and metastatic (n = 5) lymph nodes. DAPI, blue. c, Quantitation of non-tumour area (NT) and tumour area (T) of metastatic LNs (n = 5). Scale bars = 636 µm. d, Representative immunofluorescence staining of ICAM-1 (red) in an uncompressed and compressed inguinal lymph node DAPI, blue. Scale bar = 1272 µm. 3 images were taken per group and are quantified in e. f, Percentage (of total CFSE + cells) of CD3 + CFSE + cells in animals receiving adoptively transferred splenocytes. n = 7 biological replicates. Statistical analysis: Data plotted as the mean±s.e.m. Significance tested by 2-tailed unpaired (b,e) and paired Students’ t-test (c).
