Extended Data Fig. 1: Lack of CD8 T-cell infiltration into the metastatic lesion.
From: Solid stress impairs lymphocyte infiltration into lymph-node metastases
a, Representative (out of about 13 images) image of immunofluorescent staining of CD8 T cells (red) in a metastatic lymph node of a patient with head and neck squamous cell carcinoma (HNSCC). Cancer cells were stained with anti-cytokeratin (green). The dashed line indicates the margin of the lymph-node lesion. Scale bar, 636 µm. b, Quantification of the area of CD8 T cells within the metastatic tumour (T) and the adjacent non-tumour (NT) lymph-node area in LN-bearing HNSCC (n = 13). c, Representative (out of about 5 images) images of immunofluorescent staining of CD8 T cells (red) in metastatic lymph nodes of mice bearing mouse 4T1 cancer cells. Cancer cells were stained with anti-cytokeratin (green). The dashed line indicates the margin of the lymph-node lesion. Scale bar, 636 µm. d, Quantification of the area of CD8 T cells in LNs bearing 4T1 cells within the non-tumour area (NT) and tumour area (T) (n = 5). Data plotted as mean±s.e.m. Statistical significance was tested via a 2-tailed paired Students’ t-test. DAPI (blue) stains all nucleated cells.
