Extended Data Fig. 1: Quantitative analysis of tumour-infiltrating CD103+ DCs, and ex vivo immunofluorescence staining of OVA+ CD103+ DCs in tumour marginal tissue. | Nature Biomedical Engineering

Extended Data Fig. 1: Quantitative analysis of tumour-infiltrating CD103+ DCs, and ex vivo immunofluorescence staining of OVA+ CD103+ DCs in tumour marginal tissue.

From: Systemic immune responses to irradiated tumours via the transport of antigens to the tumour periphery by injected flagellate bacteria

Extended Data Fig. 1

a, Fold change of the intratumoural MHCII+CD11c+CD103+ DCs compared with RT + dB+ treatment 72 h post-injection of antigen-capturing bacteria (bacteria, 5·106 CFU per mouse; OVA-FITC, 5 mg·kg−1; n = 5, biologically independent animals). b, Ex vivo immunofluorescence staining of OVA+ CD103+ DCs in distal tumour marginal tissue. The images of tumour marginal tissue were captured 12 h after intratumoural injection of the antigen-capturing bacteria (5·106 CFU per mouse; OVA-FITC, 2.5 mg·kg−1). OVA, DCs, and tumour nuclei were stained with FITC (green), anti-CD11c-PE antibody (yellow), anti-CD103-APC antibody (red), and DAPI (blue), respectively. Scale bar, 30 μm. c, Semiquantitative analysis of the OVA distribution in distal tumour margin (n = 5, biologically independent animals).

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