Extended Data Fig. 4: Differential expression analysis of proteases in ICB response and resistance. | Nature Biomedical Engineering

Extended Data Fig. 4: Differential expression analysis of proteases in ICB response and resistance.

From: Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors

Extended Data Fig. 4

a, Heatmaps showing row-normalized expression (FPKM) of proteases differentially expressed between αPD1-treated WT tumours and IgG1-treated controls (n = 5 biological replicates). b, (Left) Volcano plots summarizing differentially expressed proteases between αPD1-treated B2m−/− and Jak1−/− MC38 tumours (n = 5 biological replicates). The threshold for differentially expressed genes (opaque dots) was defined as P value ≤ 0.05 and |log2(fold change)| ≥ 1. (Right) Heatmaps showing row-normalized expression (FPKM) of proteases differentially expressed between B2m−/− and Jak1−/− MC38 tumours (n = 5 biological replicates). c, (Left) Volcano plots summarizing differentially expressed proteases between human tumours from responders (CR + PR) and non-responders (PD) (n = 5 independent patient samples). The threshold for differentially expressed genes was defined as P value ≤ 0.01 and |log2(fold change)| ≥ 1. (Right) Heatmaps showing row-normalized expression (FPKM) of proteases differentially expressed between human tumours from responders (CR + PR) and non-responders (PD) (n = 5 independent patient samples).

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